Analytical phytochemistry
Hit Generation/Lead identification from Natural product
• Development of analytical methods for chemical analysis (metabolic profiling of bioactive natural product extracts) using high performance liquid chromatography mass spectrometry.
• Identification, estimation and structural characterization of bioactive principal compounds in herbs/extracts.
• Development of isolation methods for biologically active compounds.
• Development of tandem mass spectrum (MS/MS) database www.tmsdatabase.org of previously isolated bioactive compounds (secondary metabolite) from natural products.
Mass Spectrometry-Based Research
• LC-MS/MS based dereplication approach for discovering New Chemical Entities (NCEs) from unexplored natural products.
• Discovery of known bioactive compounds from previously unknown sources.
• Distribution of known compounds in different biological source (Plants/herbs).
• Identification of known/new derivatives of bioactive compounds in different biological source (Plants/herbs).
• Stereochemical differentiation by metal cationization tandem mass spectrometric studies. Effect of transition metal chlorides complexation on collision induced dissociation of small molecules.
Analytical phytochemistry
Bioactivity guided fractionation and HPLC/LC-MS chemical profiling to ensure chemical composition of active fraction.
Chemical Fingerprint of bioactive fraction by LC-MS/MS
Identification of New calotropogenin glycosides from Calotropis procera
Identification of known/new derivatives of bioactive compounds in different biological source (Plants/herbs)