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Main Areas of Activity

The EM Unit at CSIR-CDRI houses two major microscopy facilities –

(i) JEOL JEM1400 Transmission Electron Microscope
(ii) FEI Quanta 250 Scanning Electron Microscope

These facilities are primarily used for various R&D projects within the institute and occasionally by external users. The EM Unit participates and contributes significantly to all disease areas of the institute by using its expertise in electron microscopy to carry out ultra-structural studies and sub-cellular localization of macromolecules of biological interest.

Our state of the art facility offers expertise in electron microscopy (TEM/SEM), from designing experiments, specimen preparation to image analysis and interpretation, for a wide variety of biological samples. We have experience with a full spectrum of biological specimens /applications along with excellent resources for both research and training.

We invite national and international collaborations and are happy to be able to provide support for industrial, government and academic research. Skill development/training programs and workshops are also organized by this Unit.


Dr. Kalyan Mitra, Principal Scientist and In-charge EM Unit
• Ms. Garima Pant, Technical Officer
• Mr. Jeevan Prakash Pandey, Technical Officer
•Mrs. Madhuli Srivastava, Senior Technician

Research Fellows

•Mr. Rohit Sahai, DBT-JRF
•Mrs. Sweta Padma Majhi, CSIR-SRF
•Mr. Rakesh Sharma, CSIR-SRF
•Ms. Nisha Kumari Singh, UGC-SRF
•Ms. Juhi Sharma, INSPIRE Fellow


We also offer the following services to external users on payment basis:

Services offered for TEM/ SEM

Charges (Rs.) Private industries/Academic/R & D Institutes
TEM (Model: Jeol JEM1400 /120 kV)
Negative staining (per grid)
Viewing grids / imaging (per hr)

SEM (Model: FEI Quanta 250)
Specimen Preparation per sample
Viewing per hr


• 18% GST extra charged.
• Service charges are payable in advance by crossed bank draft in favour of the Director, Central Drug Research Institute, Lucknow-226031.
• Prior booking/appointment has to be confirmed by writing to Dr. K. Mitra (In-charge EM Unit) at

Request Form (For External users)

➤  1. Link for TEM Request Form

➤  2. Link for SEM Request Form

Major Equipment and Instrumentation

Transmission Electron Microscope

Model: Jeol JEM 1400 (Jeol Ltd., Tokyo, Japan)

• Easily changeable accelerating voltages from 40-120 KeV
• Tungsten emitter; motorized goniometer
• Magnification up to 300000X
• Equipped with Gatan Erlangshen and Orius CCD cameras
• Anti-contaminator device

High resolution /high contrast lens system; Minimum/low dose imaging for radiation sensitive samples; option for LaB6 emitter; tomography

Resolution: Point: 0.38 nm/lattice: 0.2 nm

Nano-characterization; Negative staining TEM; ultra-structural studies of biological specimens

Electron Microscopy Unit

Scanning Electron Microscope

Model: FEI Quanta 250 (FEI, The Netherlands)

• High Tension 200V – 30 kV; TMP pumped system
• Tungsten emitter
• 2 inch/50 mm 4-axis motorized stage equipped with through-the-lens differential pumping
• Detectors: SE; BSED and LFD
• High vacuum (HV) and low vacuum (LV) modes
• Magnification up to 1000000X

Multi-stub holder can hold upto 7 specimens; Provision for observation of samples with minimum sample prep.

Resolution: 3 nm at 30 kV with SE and 4 nm with BSE detector (HV mode)

Surface morphology analysis of all kinds of conductive and non-conductive samples.


Recent Selected Publications

Subhashis Pal, Mohd Sayeed, Amit Kumar, Devesh P Verma, Munesh K Harioudh, Neeraj K Verma, Konica Porwal, Shivani Sharma, Chirag Kulkarni, Amitabha Bandyopadhyay, Madhav N Mugale, Kalyan Mitra, Jimut K Ghosh, Naibedya Chattopadhyay Self-Assembling Nano-Globular Peptide from Human Lactoferrin Acts as a Systemic Enhancer of Bone Regeneration: A Novel Peptide for Orthopedic Application. ACS Appl Mater Interfaces. 2021 Apr 21;13(15):17300-17315.
Kumari T, Verma DP, Afshan T, Verma NK, Pant G, Ali M, Shukla PK, Mitra K, Ghosh JK. A Noncytotoxic Temporin L Analogue with In Vivo Antibacterial and Antiendotoxin Activities and a Nonmembrane-Lytic Mode of Action. ACS Infect Dis. 2020 Sep 11;6(9):2369-2385.
Rohit Sahai, Arindam Bhattacharjee, Vishwa Nath Shukla, Pragya Yadav, Mohammad Hasanain, Jayanta Sarkar, T. Narender, Kalyan Mitra*. Gedunin isolated from the mangrove plant Xylocarpus granatum exerts its anti-proliferative activity in ovarian cancer cells through G2/M-phase arrest and oxidative stress-mediated intrinsic apoptosis. Apoptosis. 2020 Aug;25(7-8):481-499.
Bhattacharjee A, Hasanain M, Kathuria M, Singh A, Datta D, Sarkar J*, Mitra K*.Ormeloxifene-induced unfolded protein response contributes to autophagy-associated apoptosis via disruption of Akt/mTOR and activation of JNK. Scientific Reports. 2018 Feb 2;8(1):2303.
Awasthi BP, Mitra K*. In vitro leishmanicidal effects of the anti-fungal drug natamycin are mediated through disruption of calcium homeostasis and mitochondrial dysfunction. Apoptosis. 2018 Aug;23(7-8):420-435
BhanuPriyaAwasthi, Manoj Kathuria, Garima Pant, NeemaKumari and Kalyan Mitra*. Plumbagin, a plant-derived naphthoquinone metabolite induces mitochondria mediated apoptosis-like cell death in Leishmaniadonovani: an ultastructural and physiological study. Apoptosis 2016,27315817
AshutoshRaghuvanshi, Ajay Kumar Jha, ManojKathuria, BhanuPriyaAwasthi, Deepak Purohit, Kalyan Mitra* and AtulGoel*. New visible light excitable donor–acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovani. Journal of Mat.Chem B. 2017,5, 2580-2587.
Hasanain M, Bhattacharjee A, Pandey P, Ashraf R, Singh N, Sharma S, Vishwakarma AL, Datta D, Mitra K*, Sarkar J*. α-Solanine induces ROS-mediated autophagy through activation of endoplasmic reticulum stress and inhibition of Akt/mTOR pathway. Cell Death Dis. 2015 Aug 27;6:e1860. doi:10.1038/cddis.2015.219. PubMed PMID: 26313911.
Bhaskar, Mitra K, Kuldeep J, Siddiqi MI, Goyal N. The TCP1γ subunit of Leishmania donovani forms a biologically active homo-oligomeric complex. FEBS J. 2015 Dec;282(23):4607-19. doi: 10.1111/febs.13521. Epub 2015 Oct 26. PubMed PMID:26395202. 
Kathuria M, Bhattacharjee A, Sashidhara KV, Singh SP, Mitra K*. Induction of mitochondrial dysfunction and oxidative stress in Leishmania donovani by orally active clerodane diterpene. Antimicrob Agents Chemother. 2014 Oct;58(10):5916-28. doi: 10.1128/AAC.02459-14. Epub 2014 Jul 28. PubMed PMID: 25070112; PubMed Central PMCID: PMC4187897 
Goel A*, Sharma A, Kathuria M, Bhattacharjee A, Verma A, Mishra PR, Nazir A, Mitra K*. New fluoranthene FLUN-550 as a fluorescent probe for selective staining and quantification of intracellular lipid droplets. Org Lett. 2014 Feb 7;16(3):756-9. doi: 10.1021/ol403470d. Epub 2014 Jan 10. PubMed PMID: 24410145.