|
Model
|
Assay system
|
Disease
|
|
FRET assays for screening of small anti-cancer molecules
|
Assay
|
Anti -cancer |
|
MTT assay
|
Assay
|
Anti -cancer |
|
Sulphorhodamine B assay (SRB)
|
Assay |
Anti -cancer
|
|
Trypan Blue exclusion
|
Assay
|
Anti -cancer
|
|
BRDU incorporation assay
|
Assay
|
Anti -cancer |
|
Anexin V/PI assay
|
Assay
|
Anti -cancer
|
|
Real-time PCR-based methods for specific apoptotic/survival gene expressions
|
Assay
|
Anti -cancer
|
|
Western based methods for specific apoptotic/survival gene expressions
|
Assay
|
Anti-Cancer
|
| Trypan Blue exclusion |
Assay
|
Anti -cancer
|
|
Anexin V/PI assay
|
Assay
|
Anti -cancer
|
| pTK-(C/EBP)-Luc assay
|
Assay
|
Anti -cancer
|
| Real-time PCR-based methods for specific apoptotic/survival gene expressions
|
Assay
|
Anti -cancer
|
| Western based methods for specific apoptotic/survival gene expressions
|
Assay
|
Anti -cancer
|
| Cytospin based Giemsa staining for assessment of differentiation
|
Assay
|
Anti -cancer
|
| FACS based assay for analysis of differentiation and Apoptosis
|
Assay
|
Anti -cancer
|
| Gelshift Assay
|
Assay
|
Anti -cancer
|
| In-vivo ubiquitination Assay
|
Assay
|
Anti -cancer
|
| Crystal violet Assay for assessment of cell growth
|
Assay
|
Anti -cancer
|
| Patient derived primary cultures for drug screening
|
Assay
|
Anti -cancer
|
| In vitro cell based assays for screening of nuclear receptors |
Assay
|
Anti -cancer
|
| standardized.Proteomic screening of anticancer agents |
Assay
|
Anti -cancer |
| Wound healing assay |
In vitro |
Cancer |
|
Migration and invasion assays
|
In vitro
|
Cancer |
| DMBA-induced mammary tumor model |
In vitro
|
Breast cancer |
| Topoisomerase inhibition assay |
In vitro
|
Cancer |
| Anti-proliferation Assay in Primary cell lines |
In vitro screening for the compounds anti-proliferation activity
|
Cancer |
| Apoptosis assay in primary cells/ tumors
-To evaluate the anti-cancerous properties of a particular compound using apoptosis assay |
In vitro/ In vivo
|
Cancer |
| DNMT, HDAC, HAT activity assays
- Epigenetic assays for aging and anti-cancer research.
Assays being used to evaluate the epigenetic targets of novel compounds in mammalian cell lines. The major epigenetic targets are DNMTs, HATs and HDACs. |
In vitro
|
Cancer, Aging |
| Telomerase assay |
In vitro
|
Cancer, Aging |
| Chromatin Immuno Precipitation (ChIP) assay- For DNA-protein interaction studies |
In vitro
|
Cancer / Male Reproduction/Neurobiology |
| Comet Assay
-For DNA damage |
In vitro
|
Cancer, Male reproduction |
| Transcription assay |
In vitro
|
Cancer, Male reproduction
|
| Clonogenic assay |
In vitro
|
Cancer |
| Proteasome inhibition assay –For screening of proteasome inhibitors |
In vitro
|
Cancer, Male reproduction |
| Mammalian two hybrid assay- Cellular function |
In vitro
|
Cancer, Male reproduction |
| Flowcytometric TUNEL assay-DNA damage, apoptosis |
In vitro
|
Cancer, Male reproduction |
| PMN Phagocytosis assay- For screening of Phagocytosis |
In vitro
|
Cancer, Male reproduction |
| Flurometry based autophagy assay- For screening of Autophagy |
In vitro
|
Cancer, Male reproduction |
| Luciferase reporter assay- For evaluation of Transcriptional activity |
In vitro
|
Cancer / Male Reproduction/Neurobiology |
* Minimum Quantity of the compound required, running cost of the assay and time required for the assay shall be communicated on request