Researchers are invited to make use of the assays detailed below.
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Model
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Assay System
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Disease
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Collagen induced platelet aggregation IC50 determination
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In vitro assay
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CVS/Anti-platelet activity
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ADP induced platelet aggregation IC50 determination
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In vitro assay
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CVS/Anti-platelet activity
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Arachidonic acid induced platelet aggregation IC50 determination
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In vitro assay
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CVS/Anti-platelet activity
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Thromboxane A2 agonist (U46619) induced platelet aggregation IC50 determination
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In vitro assay
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CVS/Anti-platelet activity
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Thrombin time IC50 determination
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In vitro assay
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CVS/Anti-coagulant activity
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Prothrombin time IC50 determination
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In vitro assay
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CVS/Anti-coagulant activity
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Activated partial thromboplastin time IC50 determination
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In vitro assay
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CVS/Anti-coagulant activity
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Inhibition of adipogenesis
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In vitro cell based assay
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CVS/Anti-dyslipidemic
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Dexamethosone induced insulin resistance model in 3T3 L1 adipocyte-
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In vitro cell based assay
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CVS/Anti-dyslipidemic
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Chronic insulin model in 3T3 L1 adipocyte
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In vitro cell based assay
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CVS/Anti-dyslipidemic
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Endothelial cell assays
• Tubulogenesis Assay
• Aortic ring assay
• 3D fibrin assay
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In vitro cell based assay
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CVS/ Ischemia
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In vitro screening for Neuroprotective activity (PD)
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In vitro cell based assay
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CNS/ Neuroprotective
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Screening for selective novel ligands for GPCRs implicated in CNS diseases.
Following receptors are available for screening
• Dopamine family: D1-D5
• Serotonin family: 5-HT1A/B/D, ,5-HT2A/B/C; 5-HT6,
• Orphan GPCRs (GPR40, GPR120, GPR109A, TGR5)
• Muscarinic Receptors (M1-5)
• Histamine Receptor: H1-H3
• Opioid receptors: KOR DOR, MOR,
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In vitro cell based assay
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CNS/ GPCRs
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Vasoreactivity EC50 determination
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In vitro Acetylcholine induced Vasorelaxation/ Phenylephrine induced vasoconstriction activity
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CVS/Vasorelaxant/ vasoconstriction activity
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Anti-inflammatory activity
• COX-1/COX2 (kit based)
• TNFα Inhibition (kit based)
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In vitro cell based assay
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Other disorder Anti-inflammatory
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* Minimum Quantity of the compound required, running cost of the assay and time required for the assay shall be communicated on request
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Model
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Disease
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Requirements
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Spontaneously hypertensive rats Continuous BP and HR recording by Telemetry in conscious SHR/SD rat
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CVS/ hypertension
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Provided on request
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Spontaneously hypertensive rats – For BP monitoring by non invasive blood pressure monitoring
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CVS/ hypertension
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Provided on request
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Monocrotaline induced pulmonary hypertension
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CVS/ Pulmonary hypertension
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Provided on request
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Collagen-adrenaline induced thrombosis (Single dose)
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CVS/Anti-thrombotic
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Provided on request
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Bleeding Time (Single dose)
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CVS/Anti-thrombotic
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Provided on request
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FeCl3 induced Thrombosis (Single dose)
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CVS/Anti-thrombotic
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Provided on request
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Arterio-Venous Shunt Thrombosis (Single dose)
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CVS/Anti-thrombotic
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Provided on request
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Anti-ischemic potential in myocardial infarction model in rats; Single dose
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CVS/ anti-ischemic
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Provided on request
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Endothelial cell assays
• In vivo matrigel angiogenesis assay
• Ear Angiogenesis in mice
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CVS/ Ischemia
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Provided on request
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Animal models for learning and memory
• Active and Passive Avoidance test/Morris Water Maze test
• Inhibition of Acetylcholinesterase activity
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CNS/Learning and memory
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Provided on request
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Parkinson’s models (6-OHDA induced rat model of Parkinson’s disease)
• Assessment of behavioural deficits by neurobehavioral tests
• Open field activity
• Rotarod
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Neurodegeneration
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Provided on request
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Anti depressant activity evaluation in CUS Model
• Tail suspensionTest
• Forced swim test
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CNS/ Anti-anxiety
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Provided on request
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Hyperlipidemic guinea model
• LDL Cholestrol
• HDL Cholestrol
• Triglycerides
• Total Cholestrol
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CVS/anti-dyslipidemic
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Provided on request
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Atherothrombosis in dyslipidemic hamsters (3 months fed on HFD)
• FeCl3 induced thrombosis
• Plasma lipids
• Expression of inflammatory cytokines
• Coagulation cascade parameters
• Platelet adhesion
• Platelet aggregation
• Vasoreactivity
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CVS/anti-dyslipidemic
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Provided on request
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Anti-inflammatory activity. Carrageenan induced paw edema in rat/mice
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Anti-inflammatory
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Provided on request
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